In the microscopic realms of scientific exploration and industrial manufacturing, every precise capture of detail has the potential to push the boundaries of technology. However, traditional microscopes often become bottlenecks when dealing with complex samples or dynamic observations, due to slow focusing speeds and insufficient precision. The laser autofocus microscopy system redefines imaging efficiency and precision through its innovative “active sensing and intelligent adjustment” technology, emerging as a powerful imaging tool in biomedical research, materials science, semiconductor inspection, and related fields.

Laser Beam Emission and Scanning: The system utilizes the self-focusing properties of the laser beam to form a point light source through the illumination pinhole, enabling point-by-point or line-by-line scanning of the sample surface. The laser beam is reflected by a beam splitter to the objective lens, where it is focused onto the sample to ensure high-precision positioning.

Optical Signal Reception and Conversion: After laser irradiation, the reflected or emitted optical signals are refocused through the objective lens and imaged at the detection pinhole. A photomultiplier tube (PMT) or a charge-coupled device (cCCD) positioned after the detection pinhole sequentially receives the light signals at each point and converts them into electrical signals.

Image Processing and Defocus Quantification: The electrical signals are transmitted to a computer, where image processing algorithms analyze the shape of the light spot. When the distance between the microscope objective and the sample surface changes, the shape of the light spot changes accordingly. The algorithm converts the spot morphology into the defocus amount of the objective lens, representing the distance deviation between the lens and the sample surface.

Autofocus Drive and Adjustment: Based on the defocus amount, the system generates pulse signals to control motor movement, driving the objective lens focusing module to adjust the lens position. Through a closed-loop feedback mechanism, the laser sensor module continuously monitors focal length changes, ensuring the objective lens rapidly and precisely reaches the focal position to achieve real-time autofocus.

High-Precision Imaging and Detection: The system incorporates an APO objective lens assembly delivering superior optical performance. Combined with a coaxial light source providing uniform and stable illumination, an industrial camera captures sharp images. This achieves micron-level detection accuracy, meeting the demands of high-precision experiments and industrial inspection applications.

If you're seeking a high-precision, high-efficiency microscopic imaging solution, the laser autofocus microscope system is undoubtedly your ideal choice! Contact us today to receive a customized solution and usher in a new era of “intelligent imaging” in the microscopic world!